The video shows the suspect, identified as 25-year-old Alek Minassian, pointing an object at cops while shouting 'kill me' before he is bundled to the floor and arrested.
Minassian is suspected of deliberately driving a van into crowds in Toronto.
While details are scarce, Canadian Public Safety Minister Ralph Goodale said he does not believe the attack was linked to terrorism. Federal mounted police are not involved in the investigation, also making a terror connection unlikely.
Multiple reports have suggested mental illness as a likely cause. It also appears Minassian had online discussions about Elliot Rodger, the Santa Barbara mass killer.
The name Minassian, or its alternative spelling Minasyan, is an Armenian name and more than 90 per cent of Armenians are Christians.
Footage has emerged showing an intense standoff between a Toronto police officer and the suspect behind the horror van rampage that left 10 dead and 15 injured on Monday
The video shows the suspect, identified as 25-year-old Alek Minassian, pointing an object at police on the street after getting out of a white van on Monday
Moments later the footage, which was shot by a witness in a nearby car, shows the suspect lying face-down on the sidewalk as the police officer handcuffs him
Police confirmed that 25-year-old Alek Minassian, of Richmond Hill, was arrested over the attack on Monday afternoon
In the video, an officer can be heard repeatedly shouting: 'Get down or you'll be shot' as the suspect continued to wave around the object.
Minassian is heard yelling 'kill me', while gesturing to the officer that he should shoot him.
Oblivious bystanders can be seen in the footage emerging from a building near Minassian before hurrying away as the standoff continued.
Moments later the footage, which was shot by a witness in a nearby car, shows the suspect lying face-down on the sidewalk as the police officer handcuffs him.
Police have since confirmed that Minassian did not have a gun. It is not clear what he was pointing at the officer.
Alek Minassian, 25, of Richmond Hill, was arrested on Monday after allegedly killing 10 people and injuring 15 others in Toronto
Mike McCormack, President of the Toronto Police Association, praised the arresting officer for his bravery, saying he made a 'continual threat assessment' before deciding that use of deadly force was not necessary.
His arrest came soon after he allegedly drove a rented van onto a crowded sidewalk on Yonge Street and started crashing into pedestrians.
Witnesses said the driver was moving fast and appeared to be acting deliberately.
Ali Shaker, who was driving southbound on Yonge Street at the time of incident, told CTV that the van sped along a sidewalk, hitting at least one stroller and 'crumbling down people one by one.'
Speaking at a news conference Monday night, Toronto Police Chief Mark Saunders raised the initial death toll of nine to 10, saying another victim had died at a hospital. He said 15 others were hospitalized.
Police said the suspect had not been known to police previously.
Asked if there was any evidence of a connection to international terrorism, the police chief said: 'Based on what we have there's nothing that has it to compromise the national security at this time.'
A senior national government official said earlier that authorities had not turned over the investigation to the Royal Canadian Mounted Police, a sign that investigators believed it unlikely terrorism was the motive.
Authorities released few details in the case, saying the investigation was still underway, with witnesses being interviewed and surveillance video being examined.
Minassian, who attended Seneca College, was described by former classmates as a socially awkward tech expert, the Globe and Mail reports.
Others described him as having a social or mental disability.
One classmate said that when he knew Minassian he couldn't drive and didn't know how to work a steering wheel.
His arrest came soon after Minassian allegedly drove a rented van onto a crowded sidewalk on Yonge Street and started crashing into pedestrians
from the sidewalk where 10 people were killed Monday
Tarpaulins were used to cover some of the bodies until they could be removed from the scene
A bystander breaks down in tears after seeing the aftermath of Monday's van attack
The van used in the attack is seen near the busy intersection where it mounted the pavement, before striking pedestrians
A woman struggles to hold back her emotions after witnessing the van attack
Minassian attended a high school program for students with special needs where he would often walk the halls with his head down and hands tightly clasped, according to former classmates.
Shereen Chami, one of his ex-classmates, told Reuters that he was not violent.
She said Minassian was part of a program at Thornlea Secondary School, in Toronto's northern suburbs, for high school students with special needs, attending a mix of mainstream and separate classes.
Chami remembers him walking the halls with his hands together and his head down, and making meowing noises.
The incident occurred as Cabinet ministers from the major industrial countries were gathered in Canada to discuss a range of international issues in the run-up to the G7 meeting near Quebec City in June.
Canadian Public Safety Minister Ralph Goodale called the incident a 'horrific attack' and said the G7 foreign ministers extended their condolences.
The driver was heading south on busy Yonge Street around 1.30pm and the streets were crowded with people enjoying an unseasonably warm day when the van jumped onto the sidewalk.
Ali Shaker, who was driving near the van at the time, told Canadian broadcast outlet CP24 that the driver appeared to be moving deliberately through the crowd at more than 30 mph.
Witnesses said the driver was moving fast and appeared to be acting deliberately, but police said they did not yet know the cause or any possible motive
A police officer stands next to a victim of an incident where a van struck multiple people at a major intersection in Toronto's northern suburbs
Firefighters stand near a covered body after a van struck multiple people at a major intersection northern Toronto
'He just went on the sidewalk,' a distraught Shaker said. 'He just started hitting everybody, man. He hit every single person on the sidewalk. Anybody in his way he would hit.'
Witness Peter Kang told CTV News that the driver did not seem to make any effort to stop.
'If it was an accident he would have stopped,' Kang said. 'But the person just went through the sidewalk. He could have stopped.
This person was intentionally doing this, he was killing everybody,' another witness at the scene said.
'He kept going, he kept going. People were getting hit, one after another.'
He added that many of the victims had been elderly while he saw a stroller fly into the air.
Toronto Police Chief Mark Saunders speaks during a news conference in Toronto
Farzad Salehi consoles his wife, Mehrsa Marjani, who was at a nearby cafe and witnessed the aftermath when a van plowed down a crowded sidewalk in Toronto
Bouquets of flowers are placed at a makeshift memorial to the victims as a woman writes her condolences after a van mounted a sidewalk crashing into pedestrians in Toronto